.MicroRNA-124 Regulates Neuronal Differentiation of Mesenchymal Stem Cells by Targeting Sp1 mRNA

.MicroRNA-124 Regulates Neuronal Differentiation of Mesenchymal Stem Cells by Targeting Sp1 mRNA


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علوم پزشکی اراک
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نویسندگان: قاسم مسیبی , بهزاد خوانساری نژاد

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نشریه: Journal of Cellular Biochemistry, 6,116,943 - 953,2015

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کد مقاله 2939
عنوان فارسی مقاله .MicroRNA-124 Regulates Neuronal Differentiation of Mesenchymal Stem Cells by Targeting Sp1 mRNA
عنوان لاتین مقاله .MicroRNA-124 Regulates Neuronal Differentiation of Mesenchymal Stem Cells by Targeting Sp1 mRNA
نوع مقاله بر حسب نگارش پژوهشی اصیل
مقاله برحسب نمایه ISI
IF 3.263
عنوان نشریه Journal of Cellular Biochemistry
نوع نشریه علمی پژوهشی
شماره نشریه 6
دوره 116
صفحه شروع و پایان در نشریه 943 - 953
سال انتشار/ ارائه شمسی 1394
سال انتشار/ارائه میلادی 2015
آدرس لینک مقاله/ همایش در شبکه اینترنت http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=10&SID=X2pPH4JUgae8fiyNbav&page=1&doc=1
ISSN 0730-2312
DOI DOI: 10.1002/jcb.25045
آدرس علمی (Affiliation) نویسنده متقاضی Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran

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چکیده انگلیسیMicroRNA-124 regulates neuronal differentiation of mesenchymal stem cells by targeting Sp1 mRNA. Mondanizadeh M1, Arefian E, Mosayebi G, Saidijam M, Khansarinejad B, Hashemi SM. • Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran • Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran • Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran • Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran • Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran • Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Abstract MicroRNAs play an important role in neuronal development and function. miR-124 is the most abundantly expressed miRNA in the nervous system. Several different mRNA targets have been proposed for miR-124, but the precise function of endogenous miR-124 and its mRNA targets remain to be further elucidated. Specificity protein 1 (Sp1) is a transcription factor that plays key roles in many cell processes including cell cycle. However, this transcription factor is nearly absent in differentiated neurons and a remarkable suppression of Sp1 expression was shown after neurogenesis. Since miR-124 is expressed abundantly in neurons and because Sp1 levels decrease during neurogenesis, it is possible that miR-124 could regulate the expression of Sp1 during neuronal development. Therefore, the aim of the present study was to evaluate the putative targeting of Sp1 by miR-124. Overexpression of miR-124 using a plasmid coding for pri-miR-124 in HEK293 cells decreased the expression of Sp1 mRNA. The results of dual-luciferase reporter assay demonstrated that miR-124 directly targeted the 3'-untranslated regions of Sp1 mRNA. To evaluate whether Sp1 expression was regulated by miR-124 during the process of neuronal differentiation, Adipose-derived mesenchymal stem cells (A-MSCs) were differentiated into neuron-like cells. The results of qPCR analysis showed that with the gradual increase of miR-124 expression during neurogenesis, the expression of Sp1 mRNA decreased accordingly. In summary, this study demonstrated for the first time that miR-124 is able to suppress Sp1 expression, which in turn affected the neuronal differentiation of mesenchymal stem cells. KEYWORDS: CELL DIFFERENTIATION; MicroRNAs; NEUROGENESIS; SPECIFIC PROTEIN
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نویسنده نفر چندم مقاله
قاسم مسیبیسوم
بهزاد خوانساری نژادپنجم

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