.In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein

.In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein


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علوم پزشکی اراک
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نویسندگان: حمید ابطحی , احسان اله غزنوی راد , لیلا فرهنگ نیا

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نشریه: Jundishapur Journal of Microbiology, 3,9,1 - 5,2016

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کد مقاله 3303
عنوان فارسی مقاله .In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein
عنوان لاتین مقاله .In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein
نوع مقاله بر حسب نگارش پژوهشی اصیل
مقاله برحسب نمایه ISI
IF 0.387
عنوان نشریه Jundishapur Journal of Microbiology
نوع نشریه علمی پژوهشی
شماره نشریه 3
دوره 9
صفحه شروع و پایان در نشریه 1 - 5
سال انتشار/ ارائه شمسی 1394
سال انتشار/ارائه میلادی 2016
آدرس لینک مقاله/ همایش در شبکه اینترنت http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=2008-3645
ISSN 2008-4161
DOI DOI: 10.5812/jjm.28489
آدرس علمی (Affiliation) نویسنده متقاضی Department of Microbiology and Immunology, Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, IR Iran

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چکیده انگلیسیIn Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein ________________________________________ Hamid Abtahi 1 ; Leila Farhangnia 2 ; and Ehsanollah Ghaznavi-Rad 3, * 1 Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, IR Iran 2 Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, IR Iran 3 Department of Microbiology and Immunology, Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, IR Iran *Corresponding author: Ehsanollah Ghaznavi-Rad, Department of Microbiology and Immunology, Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, IR Iran. Tel/Fax: +98-8634173526, E-mail: e.ghaznavirad@arakmu.ac.ir. Jundishapur Journal of Microbiology. 9(3): e28489 , DOI: 10.5812/jjm.28489 Article Type: Research Article; Received: Mar 7, 2015; Revised: May 31, 2015; Accepted:Jun 14, 2015; epub: Mar 2, 2016; collection: Mar 2016 Abstract Background: Bacterial infection by antibiotic-resistant Staphylococcus aureus strains is a worldwide concern and the development of novel antistaphylococcal agents is acutely needed. Lysostaphin, an example of such novel agents, is a bacteriocin secreted by S. simulans to kill S. aureus through proteolysis of the Staphylococcus cell wall. Objectives: The aim of this study was to evaluate the in vitro and in vivo antistaphylococcal activity of recombinant lysostaphin. Materials and Methods: The in vitro study of the recombinant lysostaphin activity against S. aureus was determined by turbidimetric assay. For in vivo investigation, two groups of rats were inoculated with 1.4 × 109 CFU S. aureus. Five days after the nasal instillation of S. aureus, treatment in one of the groups was performed with a single dose (200 μg/dose) of recombinant lysostaphin formulated in Eucerin-based cream. Results: Recombinant lysostaphin at 100 μg/mL concentration showed a significant decrease of the optical density compared to the control samples. The in vivo study demonstrated that a single dose (200 μg/dose) of recombinant lysostaphin cream significantly reduced nasal colonization in all the treated animals compared to the untreated ones. Conclusions: These results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal S. aureus in rats. It can be recommended for human clinical trial studies. Keywords: Lysostaphin; Recombinant Protein; Staphylococcus aureus
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نویسنده نفر چندم مقاله
حمید ابطحیاول
احسان اله غزنوی رادسوم و مسئول
لیلا فرهنگ نیادوم

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jjm-09-03-28489.pdfمتن کامل مقاله1395/01/14158723دانلود